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low salt ip buffer  (Active Motif)


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    Structured Review

    Active Motif low salt ip buffer
    Low Salt Ip Buffer, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/low+salt+ip+buffer/low+salt+ip+buffer/pm33311559-322-28-32
    Average 90 stars, based on 1 article reviews
    low salt ip buffer - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: The SWI/SNF Chromatin Remodeling Complex Regulates Myocardin-Induced Smooth Muscle–Specific Gene Expression
    Article Snippet: Co-immunoprecipitation (Co-IP) assays were performed using a nuclear complex Co-IP kit as described by the manufacturer (Active Motif). .. 250 μg of nuclear protein extracts were incubated with 3 μg of anti-Brg1 antibody (Upstate), anti-SRF antibody (Santa Cruz, G20X), anti-myocardin antibody (Santa Cruz, M16), or appropriate IgG controls in 500μl low salt IP buffer (Active Motif), overnight at 4 0C. .. 60 μl of EZview protein A beads (Sigma) were added to the mixture for an additional 1 hour with rocking and then wash 6 times with the low IP buffer.

    Article Title: Tannic acid facilitates expression of the polypyrimidine tract binding protein and alleviates deleterious inclusion of CHRNA1 exon P3A due to an hnRNP H-disrupting mutation in congenital myasthenic syndrome
    Article Snippet: We employed the Nuclear Complex Co-IP kit (Active Motif). .. We incubated 250 μg of a nuclear protein extract of HEK293T cells with 3 μg of anti-hnRNP F/H antibody (Santa Cruz) or isotype-matched control antibody in 500 μl of a low salt IP buffer (Active Motif) overnight at 4°C. .. We added 60 μl of protein G beads (GE Healthcare) and incubated for an additional hour on a rotary shaker.

    Article Title: YY1 directly interacts with myocardin to repress the triad myocardin/SRF/CArG box-mediated smooth muscle gene transcription during smooth muscle phenotypic modulation.
    Article Snippet: 48 h after transfection, nuclear protein was harvested and Co-IP assays were performed using the nuclear complex Co-IP kit as described by the manufacturer (Active Motif, cat. #: 54,001). .. One hundred micrograms of nuclear protein extracts were incubated with 50 μl of anti-Myc beads (Sigma, cat. #: E6654) or 3 μg control rabbit IgG in 500 μl low salt IP buffer (Active Motif) overnight at 4 °C. ..

    Article Title: SOX9 and myocardin counteract each other in regulating vascular smooth muscle cell differentiation
    Article Snippet: Forty eight hours after transfection, nuclear protein was harvested and Co-IP assays were performed using the nuclear complex Co-IP kit as described by the manufacturer (Active Motif). .. One hundred micrograms of nuclear protein extracts were incubated with 50 μl of anti-Myc beads (Sigma) or 3 μg control rabbit IgG in 500 μl low salt IP buffer (Active Motif) overnight at 4 °C. ..

    Article Title: Modulation of Myocardin Function by the Ubiquitin E3 Ligase UBR5
    Article Snippet: 48 h after transduction, nuclear protein was harvested and co-IP assays were performed using a nuclear complex co-IP kit as described by the manufacturer (Active Motif). .. 250 g of nuclear protein extracts were incubated with 3 g of anti-myocardin antibody (Santa Cruz, M16, goat), UBR5 (Santa Cruz, goat), or normal goat IgG controls in 500 l of low salt IP buffer (Active Motif) overnight at 4 °C. .. 60 l of TrueBlotTM anti-goat Ig IP Beads (eBioscience) were added to the mixture for an additional 1 h with rocking and then immobilized complexes were washed 6 times with the low salt IP buffer.

    Article Title: Modulation of Myocardin Function by the Ubiquitin E3 Ligase UBR5
    Article Snippet: 48 h after transduction, nuclear protein was harvested and co-IP assays were performed using a nuclear complex co-IP kit as described by the manufacturer (Active Motif). .. 250 μg of nuclear protein extracts were incubated with 3 μg of anti-myocardin antibody (Santa Cruz, M16, goat), UBR5 (Santa Cruz, goat), or normal goat IgG controls in 500 μl of low salt IP buffer (Active Motif) overnight at 4 °C. .. 60 μl of TrueBlot TM anti-goat Ig IP Beads (eBioscience) were added to the mixture for an additional 1 h with rocking and then immobilized complexes were washed 6 times with the low salt IP buffer.

    Control:

    Article Title: Tannic acid facilitates expression of the polypyrimidine tract binding protein and alleviates deleterious inclusion of CHRNA1 exon P3A due to an hnRNP H-disrupting mutation in congenital myasthenic syndrome
    Article Snippet: We employed the Nuclear Complex Co-IP kit (Active Motif). .. We incubated 250 μg of a nuclear protein extract of HEK293T cells with 3 μg of anti-hnRNP F/H antibody (Santa Cruz) or isotype-matched control antibody in 500 μl of a low salt IP buffer (Active Motif) overnight at 4°C. .. We added 60 μl of protein G beads (GE Healthcare) and incubated for an additional hour on a rotary shaker.

    Article Title: YY1 directly interacts with myocardin to repress the triad myocardin/SRF/CArG box-mediated smooth muscle gene transcription during smooth muscle phenotypic modulation.
    Article Snippet: 48 h after transfection, nuclear protein was harvested and Co-IP assays were performed using the nuclear complex Co-IP kit as described by the manufacturer (Active Motif, cat. #: 54,001). .. One hundred micrograms of nuclear protein extracts were incubated with 50 μl of anti-Myc beads (Sigma, cat. #: E6654) or 3 μg control rabbit IgG in 500 μl low salt IP buffer (Active Motif) overnight at 4 °C. ..

    Article Title: SOX9 and myocardin counteract each other in regulating vascular smooth muscle cell differentiation
    Article Snippet: Forty eight hours after transfection, nuclear protein was harvested and Co-IP assays were performed using the nuclear complex Co-IP kit as described by the manufacturer (Active Motif). .. One hundred micrograms of nuclear protein extracts were incubated with 50 μl of anti-Myc beads (Sigma) or 3 μg control rabbit IgG in 500 μl low salt IP buffer (Active Motif) overnight at 4 °C. ..



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